Order Of Draw and Common Tests

Hey girls! Ive been in Phlebotomy school for the past 2 months and here is what I have learned about the most Common Tubes and their Tests in the order of draw!

1. Discard Tube (clear)- always draw a discard tube in routine venipuncture to prevent inaccurate test results.

2. Light Blue Tube- this tube must always be filled to capacity and is the first or second tube in order of draw depending on your facility.

3. Red Tube- have 2 different types of tubes and the non-additive red top tube can be used as a discard tube

4. Gold or Red/Black Speckled (Tiger) Top Tubes- serum separator tubes (SSTs) and these tubes help prevent chemical changes caused by cellular metabolism and fibrinolysis

5. Light Green or Green/Gray Speckled Tubes- plasma separator tubes (PST) and are used to form a barrier between blood cells and plasma

6. Green Tube- stop the coagulation process by inactivating thrombin

7. Lavender Tube- EDTA prevents blood from clotting by binding with calcium and also maintains cells shape and size

8. Pink Tube- preferred for blood banks and the label on these tubes contain information required by AABB

9. Gray Tube- contain an antiglycolytic agent (sodium fluoride) that which prevents RBCs from using glucose and changing it to lactic acid

I hope this information will help you remember and learn the order of draw and the most common tests used for each tube. I know it to me a while to get it and understand the order of draw and this definitely helped me to have it broken down into simple terms! Good luck to all you future phlebotomists 💉🩷

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